RADIOGRAPHIC ANALYSIS OF Campomanesia adamantium SEEDS TO EVALUATE THE DESICCATION SENSITIVITY
Leão-Araújo, E.F.1, Gomes-Junior, F.G.2, Peixoto, N.3 and Souza, E.R.B. de4
1Department of Agronomy, Goiano Federal Institute, Urutaí, Goiás, Brazil
l2Crop Science Department, University of São Paulo - ‘Luiz de Queiroz’ College of Agriculture, Piracicaba, São Paulo, Brazil
3Department of Agronomy, Goiás State University, Ipameri, Goiás, Brazil
4College of Agronomy, Goiás Federal University, Goiânia, Goiás, Brazil
Contact: Érica Leão-Araújo, email@example.com
Campomanesia adamantium is native to the Brazilian Savannah and presents a high potential of use, e.g., consumption of fresh fruit, medicinal plant, manufacture of sweets, ice cream and alcohol distillates. The seeds of this species are recalcitrant. Recalcitrance is a phenomenon of intolerance to dehydration and affects the seeds physiological quality. The seed desiccation can be monitored by retraction of the tissues from the integument, which is identified by the inspection of internal morphology of the seed. Radiographic images have been recommended to evaluate the internal morphology of seeds allowing to stablish a relationship with their physiological potential. Thus, this work aimed to evaluate internal morphology changes during dehydration of Campomanesia adamantium seeds by using radiographic analysis and the effects on the physiological quality. The water content of the seeds was initially determined and adjustments were made to obtain samples with 48%, 36%, 30%, 27%, 24% and 21% of water (web basis). The individual radiographs of the seeds were obtained by a digital X-ray equipment and the images evaluated using the ImageJ® software to determine the free internal space. Germination, first germination count, germination speed index, mean germination time and seedling lenght evaluations were performed. With these data, polymonial regression graphs were constructed. The X-ray images were used to obtain data of free internal space and percentage of seeds with physical injuries. Data were submitted to analysis of variance and the effect were studied through polynomial regression. The germination and vigor decreased as the seeds were dehydrated. The free internal space was higher as the water content of the seeds was reduced. The percentage of seeds with physical injuries also followed this behavior with a tendency of stabilization in water contents higher than 36%. The damage caused by the reduction of the water content as well as the increase of the retraction of the internal tissues to the seed coat had a negative effect in the seed vigor. The X-ray image analysis is efficient to determine internal free spaces during desiccation of Campomanesia adamantium seeds that are associated with reduction in their physiological quality.