Academic Staff Scientists
Stanford university
The SyneRx Center at Stanford University is one of nine National Antiviral Drug Discovery Centers for Pathogens of Pandemic Concern (AViDD) recently funded by the National Institute for Allergy and Infectious Diseases. We are seeking to appoint two Ph.D. level scientists that will bring complementary research experience in cryoEM/ET and synchrotron-enabled x-ray techniques, primarily macromolecular crystallography. Stanford’s SyneRx Center includes a Structural Biology Core (SBC) to provide state-of-the-art tools and capabilities for structural determinations from RNA and proteins to virus and virus infected cells. This SBC is comprised of three Subcores including protein production (at Stanford University campus facilities and labs), x-ray crystallography and scattering (at the Stanford Synchrotron Radiation Lightsource), and cryogenic electron microscopy and tomography (cryoEM/ET at facilities located at SLAC National Accelerator Laboratory).
As a staff scientist, you would work collaboratively to drive all aspects of the three Subcores in the SBC, specifically through interacting with scientists in the research teams and providing coordination in areas that include sample preparation and characterization, data acquisition, modeling and structure determination and validation, pipe-line organization and feedback. You would also assist and train the SyneRx Center’s Project scientists in performing the structure characterizations to facilitate and accelerate the design of novel and effective antivirals against SARS-CoV-2 and other RNA viruses of pandemic potential.
Link:
https://smb.slac.stanford.edu
Faculty Positions in Biochemistry & Molecular Biology
University of Texas Medical Branch (UTMB Health)
The Department of Biochemistry and Molecular Biology is a diverse biomedical research community with strengths in molecular virology, RNA biology, mechanobiology, structural biology, biophysics, and quantitative biology. In addition to a highly collaborative environment, UTMB offers outstanding research facilities for next-generation sequencing, mass spectrometry, optical microscopy, flow cytometry, solution biophysics, NMR, X-ray crystallography, cryo-electron microscopy, scientific computing, and bioinformatics. Excellent opportunities for scientific interactions also exist through sister institutions in the Greater Houston area, Gulf Coast Consortia, and the Keck Center for Interdisciplinary Bioscience.
An attractive recruitment package of competitive salary, generous start-up funding, and renovated laboratory space will be offered. Interested applicants should submit a single PDF (<10 MB) containing the following: 1) cover letter, 2) curriculum vitae, 3) summary of research accomplishments and future goals (2-3 pages), 4) diversity statement which follows the NIH PEDP guidelines, 5) teaching and mentoring statement (1 page) and 6) contact information for three references.
Link:
https://bmb.utmb.edu/
Postdoctoral researcher at Lund Medical Faculty
Curevac
We seek a highly motivated postdoctoral researcher to be based in the Molecular Biophysics Group within the Biomedical Centre (BMC) in the Medical Faculty of Lund University, led by Professor Trevor Forsyth. The project will be carried out in close collaboration with CureVac (https://www.curevac.com/en/ ) in Germany, and with the LSS and Life Sciences Groups at the Institut Laue Langevin (ILL - https://www.ill.eu/ ) in France. CureVac is a leading global clinical-stage biopharmaceutical company dedicated to the development of new classes of drugs based on the use of messenger RNA (mRNA) technologies. The ILL is the most intense neutron source in the world and holds a leading position for neutron science in life sciences, soft condensed matter, and hard condensed matter.The researcher will be based in the Lund Medical Faculty, with good access to state-of-the-art facilities both within the Medical, Science, and Engineering faculties, and in close proximity to the nearby MAX-IV synchrotron and ESS neutron beam facilities, as well as access to the ILL and ESRF sources in Grenoble, France.
Link:
https://lu.varbi.com/en/what:job/jobID:540560/
A researcher2 position is available at Dr. Fang Li lab UMN.
UMN
Applicants are invited to a Researcher 2 position in the Laboratory of Structural Biology of Disease at the Department of Pharmacology, Medical School, the University of Minnesota, headed by Dr. Fang Li (PI).
Basic Qualifications
· A master’s degree in Molecular Biology or related fields or a bachelor’s degree in Molecular Biology with 2+ years of relevant experience.
· Hands-on experiences include molecular cloning, mammalian cell culture, protein expression and purification.
· Innovation and self-motivation, capable of working independently and in a team setting.
Excellent verbal and written communication skills
Link:
http://www.msi.umn.edu/~lifang
Ph.D. Position
Institut de Biologie Structurale (IBS)
Structure of the vaccinia virus DNA replication machinery in complex with DNA substrates by single-particle cryo-electron microscopy
With the recent spread of monkeypox infections, poxviruses got into the headlines. Our team works on the elucidation of structure and function of the poxvirus DNA replication machinery, now mainly by single-particle cryo-electron microscopy. The DNA replication of vaccinia virus, a safe model system, involves the DNA polymerase holoenzyme complex E9-A20-D4, furthermore the hexameric helicase-primase D5. The aim is to determine structures of these assemblies by cryo-EM with bound DNA substrates, nucleotides, inhibitors and nucleotide analogues in order to stabilize different functional states for the understanding of poxvirus DNA replication. The project builds on the team’s experiences in the structure determination and biophysical characterization of the partners and their domains, our recent results on the cryo-EM of the helicase domain of D5 and first results by cryo-EM on the holoenzyme.
The candidate should be computer literate and have some experience in the use of Linux. Knowledge of relevant software packages used for cryo-EM will be an asset. Furthermore, a sound background in biochemistry, physics or biophysics is required as the project comprises multiple methods: protein production in the baculovirus-insect cell system; protein purification by affinity chromatography; sample preparation for cryo-EM. structure calculations for single-particle cryo-EM on a Linux computing cluster; analysis and visualization of protein structures.
Link:
https://www.ibs.fr/research/research-groups/viral-replication-machines-group-m-jamin/
PhD & postdoc positions
the Max Planck Institute for Multidisciplinary Sciences
a PhD student or postdocs to explore molecular mechanisms in the ubiquitin system.
We are particularly looking to recruit candidates with a background and interest in protein biochemistry and X-ray crystallography or cryo-EM
Link:
https://www.mpinat.mpg.de/4152583/34-22?c=3895698
Postdoc fellow
Dana-Farber Cancer Institute and Harvard Medical School
We use interdisciplinary approaches at the interface of structural biology and the molecular analysis of cellular immunity with the aim of translating new insights into T cell cellular immunotherapies including T cell receptor designs and rational cancer vaccine developments.
Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow’s physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.
Responsibilities
Qualifications
Highly motivated candidates with a recent PhD or MD/PhD in structural biology and significant experience in molecular biology, biochemistry, protein expression and with some background in immunology are encouraged to apply. Skills in molecular cloning, protein chemistry and crystallography and/or other structural approaches are essential. Candidates should have a publication record in peer-reviewed journals. We are seeking a candidate with excellent writing and communication skills, able to work independently but also effectively with other lab members. Applicants should include a short statement of research goals, CV and three references with their application.
Link:
None
PhD position in ribosomes modifications
Cancer Research Center of Lyon
The PhD project aims at understanding the role of 2’-O-methylation of rRNA in ribosome activity and translational regulation in human cells. The project involves cell biology, ribosome biochemistry and in vitro methods and is associated with a cryoEM study in collaboration with B. Klaholz. Data from human tumor samples and cancer cell models, and from structural studies will guide the rational engineering of specific 2’-O-methylation defective ribosomes.
This is a 3-year funding by ANR.
Student Profile
• You hold a Master degree in biology, biochemistry or molecular biology (or equivalent degree from an engineering school).
• You have a passion for understanding the structure and molecular mechanisms involved in life. Skills in cell biology or biochemistry will be key.
• Knowledge in molecular biology, in vitro translation and cell imaging would be a plus. Excellent communication skills and high motivation.
The Lab – www.crcl.fr
The “Ribosome, Translation and Cancer Team” (PI, J-J. Diaz) is located in the Cancer Research Center of Lyon and offers a dynamic environment for PhD students and post-docs. Lyon is ideally located, just a couple of hours from the Alpes, the south riviera and Paris.
Spoken languages are French and English.
Link:
www.CRCL.fr
PhD position in ribosomes and HCV
Cancer Research Center of Lyon
Dealing with the impact of HCV on the methylation of ribosomes (Coll. with JJ Diaz’s team), funded by the ANRS.
Any person interested, owning a M2 diploma in 2022, should contact us with the name of two referees, his/her CV and a simple letter of motivation. People with experience in cell translation, or HCV and/or liver virology or pathology will be welcome. Secondary expertise in bioinformatics will be a plus, although not crucial either.
Start date must be Feb 1st 2023 at the latest.
Relevant publications
Lahlali T, Et Al. Netrin-1 Protects Hepatocytes Against Cell Death Through Sustained Translation During the Unfolded Protein Response. Cell Mol Gastroenterol Hepatol. 2016 Jan 9;2(3):281-301.e9. doi: 10.1016/j.jcmgh.2015.12.011. PMID: 28174720; PMCID: PMC5042567.
Plissonnier ML, Et Al. Epidermal Growth Factor Receptor-Dependent Mutual Amplification between Netrin-1 and the Hepatitis C Virus. PLoS Biol. 2016 Mar 31;14(3):e1002421. doi: 10.1371/journal.pbio.1002421. PMID: 27031829; PMCID: PMC4816328.
Barnault R, Et Al. Hepatic inflammation elicits production of proinflammatory netrin-1 through exclusive activation of translation. Hepatology. 2022 Mar 7. doi: 10.1002/hep.32446. Epub ahead of
print. PMID: 35253915.
Therizols G, Et Al. Alteration of ribosome function upon 5-fluorouracil treatment favors cancer cell drug-tolerance. Nat Commun. 2022 Jan 10;13(1):173. doi: 10.1038/s41467-021-27847-8. PMID: 35013311; PMCID: PMC8748862.
Erales J, Et Al. Evidence for rRNA 2’-O-methylation plasticity: Control of intrinsic translational capabilities of human ribosomes. Proc Natl Acad Sci U SA. 2017 Dec 5;114(49):12934-12939. doi: 10.1073/pnas.1707674114. Epub 2017 Nov 20. PMID: 29158377; PMCID: PMC5724255.
Marcel V, Et Al. p53 acts as a safeguard of translational control by regulating fibrillarin and rRNA methylation in cancer. Cancer Cell. 2013 Sep 9;24(3):318-30. doi: 10.1016/j.ccr.2013.08.013. PMID: 24029231; PMCID: PMC7106277.
Link:
None
